enzyme engen spy cas9 hf1 (New England Biolabs)
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Enzyme Engen Spy Cas9 Hf1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cas9+enzyme/EnGen+Spy+Cas9+HF1/pmc13127573-258-2-7
Average 94 stars, based on 13 article reviews
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CRISPR:Article Title: Nonstop mutations cause loss of renal tumor suppressor proteins VHL and BAP1 and affect multiple stages of protein translation Article Snippet: The resulting 27 μl of cell/RNP solution was mixed gently, added to 1 well of the 16-well Nucleocuvette Strip and immediately electroporated with the DS-150 protocol in the 4D-Nucleofector System (Lonza #AAF-1002B with AAF-1002X). .. For the nucleofection mix for the CRISPR-Cas9 method (for BAP1 ), 2.8 μl of RNP mix was formulated by mixing 1.8 μl of 100 μM Synthego Synthetic sgRNA (dissolved in 10 mM tris and 1 mM EDTA, pH 8.0) and 1 μl of Staphylococcus pyogenes Article Title: Nonstop mutations cause loss of renal tumor suppressor proteins VHL and BAP1 and affect multiple stages of protein translation. Article Snippet: The resulting 27 μl of cell/RNP solution was mixed gently, added to 1 well of the 16- well Nucleocuvette Strip and immediately electroporated with the DS- 150 protocol in the 4DNucleofector System (Lonza #AAF- 1002B with AAF- 1002X). .. For the nucleofection mix for the CRISPR- Cas9 method (for BAP1), 2.8 μl of RNP mix was formulated by mixing 1.8 μl of 100 μM Synthego Synthetic sgRNA (dissolved in 10 mM tris and 1 mM EDTA, pH 8.0) and 1 μl of Staphylococcus pyogenes Incubation:Article Title: Nonstop mutations cause loss of renal tumor suppressor proteins VHL and BAP1 and affect multiple stages of protein translation Article Snippet: The resulting 27 μl of cell/RNP solution was mixed gently, added to 1 well of the 16-well Nucleocuvette Strip and immediately electroporated with the DS-150 protocol in the 4D-Nucleofector System (Lonza #AAF-1002B with AAF-1002X). .. For the nucleofection mix for the CRISPR-Cas9 method (for BAP1 ), 2.8 μl of RNP mix was formulated by mixing 1.8 μl of 100 μM Synthego Synthetic sgRNA (dissolved in 10 mM tris and 1 mM EDTA, pH 8.0) and 1 μl of Staphylococcus pyogenes Article Title: Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption. Article Snippet: The sgRNAs (sgRNA 1 and 2; S1 Table) were obtained from Synthego Corporation (Redwood City, California, USA). .. The sgRNAs efficacies were confirmed by an in vitro digestion; 180 pmol of each sgRNA was incubated with 20 pmol of Article Title: Nonstop mutations cause loss of renal tumor suppressor proteins VHL and BAP1 and affect multiple stages of protein translation. Article Snippet: The resulting 27 μl of cell/RNP solution was mixed gently, added to 1 well of the 16- well Nucleocuvette Strip and immediately electroporated with the DS- 150 protocol in the 4DNucleofector System (Lonza #AAF- 1002B with AAF- 1002X). .. For the nucleofection mix for the CRISPR- Cas9 method (for BAP1), 2.8 μl of RNP mix was formulated by mixing 1.8 μl of 100 μM Synthego Synthetic sgRNA (dissolved in 10 mM tris and 1 mM EDTA, pH 8.0) and 1 μl of Staphylococcus pyogenes Article Title: Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption Article Snippet: The sgRNAs (sgRNA 1 and 2; ) were obtained from Synthego Corporation (Redwood City, California, USA). .. The sgRNAs efficacies were confirmed by an in vitro digestion; 180 pmol of each sgRNA was incubated with 20 pmol of Sequencing:Article Title: CRISPR/Cas9-Mediated Genome Editing in Zebrafish Article Snippet: .. In Vitro:Article Title: Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption. Article Snippet: The sgRNAs (sgRNA 1 and 2; S1 Table) were obtained from Synthego Corporation (Redwood City, California, USA). .. The sgRNAs efficacies were confirmed by an in vitro digestion; 180 pmol of each sgRNA was incubated with 20 pmol of Article Title: Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption Article Snippet: The sgRNAs (sgRNA 1 and 2; ) were obtained from Synthego Corporation (Redwood City, California, USA). .. The sgRNAs efficacies were confirmed by an in vitro digestion; 180 pmol of each sgRNA was incubated with 20 pmol of |


